Yeah, pretty much what I suspected....
A two-generation dietary test provides a method for determining multigenerational
effects of endocrine disrupting chemicals (EDCs), with varied exposures over the stages in the
life cycle. The Japanese quail has been developed as a model for precocial avian species;
however, northern bobwhite quail have been the standard for toxicological testing. Our purpose
was to compare relevant end points in these species, in a two-generation dietary study. In
addition the to species comparison, a range of potential measurement end points were examined,
including appraisal of potential sensitivity, constraints, and reliability for regulatory application.
A modification of the OECD one generation protocol was followed with each generation for
definition of the adult experimental phase of the study. The first generation (parental generation)
was synchronized by raising both species under short photoperiod until mature. Specifically,
Japanese and northern bobwhite quail (parents; P) were raised under short photoperiod, then
placed on diet containing low concentrations of methoxychlor (MXC; 0, 5ppm, and 10ppm) and
simultaneously photostimulated. A range of measures were monitored including feed intake, egg
production, egg quality, fertility, offspring viability. In addition, other measures were selected
for their potential to detect endocrine disruption. Chicks (F1) were raised on the same diet as
their parents, paired as adults, and sexual maturation, reproductive behavior and endocrine
measures were examined, similar to the parent generation. F2 chicks were raised on control feed
and assessed for the same end points as the F1 birds.
